11582 Tomm22 Status: ES cell colonies screened / QC no positives
| Gene | Tomm22 |
| Chromosome | 15 |
| Deletion Start | 79,670,877 |
| Deletion End | 79,672,297 |
| Deletion Size | 1,421 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,671,245 | 79,671,264 | GGAGCCACCTTTGACCTCTC |
| TUP | 79,671,267 | 79,671,294 | TCTTCGTGGCTCAGAAGATGTACAGGTA |
| TUR | 79,671,347 | 79,671,365 | AGGCCCGCTGTCCTTTGAC |
| TDF | 79,671,773 | 79,671,796 | GGAATTGGTAGGGTACGCTTTTAG |
| TDP | 79,671,797 | 79,671,821 | AAGATCGAGGGCGGTACTGCACGTC |
| TDR | 79,671,822 | 79,671,841 | TAGGACCGAACCTGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | TGCAGTTTCGGAGCCAAGC |
| SD | TAAGAACTTCCTTAGGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 358 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
