11584 Pnrc2 Status: Cancelled
| Gene | Pnrc2 |
| Chromosome | 4 |
| Deletion Start | 135,872,433 |
| Deletion End | 135,871,894 |
| Deletion Size | 540 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 135,872,283 | 135,872,305 | GGAACGAGGACATGGGTACAATC |
| TUP | 135,872,257 | 135,872,279 | CAGCAGCAGCATGGCAGGCCATG |
| TUR | 135,872,204 | 135,872,224 | CCAGCATTCCAGTTGGAGTTG |
| TDF | 135,872,113 | 135,872,135 | AACCACCATCACCAAGTGTTCTC |
| TDP | 135,872,089 | 135,872,111 | CCAAGCCACCAAGCCACTGGGTT |
| TDR | 135,872,056 | 135,872,080 | CGTTTCCTTATCTGAAGGGTTCAAG |
PCR Primers
| Primer | Sequence |
| SU | AAGAATGAAAGAAGAGCTTGC |
| SD | AATGACCACTGGGACATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 344 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
