11587 Cxcl1 Status: Cancelled
| Gene | Cxcl1 |
| Chromosome | 5 |
| Deletion Start | 90,891,314 |
| Deletion End | 90,892,535 |
| Deletion Size | 1,241 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,891,705 | 90,891,725 | GCAGAGCCACACTCAAGAATG |
| TUP | 90,891,727 | 90,891,746 | TCGCGAGGCTTGCCTTGACC |
| TUR | 90,891,790 | 90,891,814 | GGTACAAACACAAGTCACAACTCAC |
| TDF | 90,892,359 | 90,892,382 | TCAAGTGATTGACTCCACAGGAAC |
| TDP | 90,892,383 | 90,892,410 | CGACAGTACAGAACTCTGGGTGCAAGTG |
| TDR | 90,892,418 | 90,892,438 | GGCTCCAGATGGGCTTTCTAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGTGGAATCCTAGGTGGC |
| SD | ACAAACACAGCCTCCCACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 532 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 443 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
