11588 Abi3 Status: ES cell colonies screened / QC positives
| Gene | Abi3 |
| Chromosome | 11 |
| Deletion Start | 95,703,457 |
| Deletion End | 95,693,941 |
| Deletion Size | 9,517 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 95,703,261 | 95,703,281 | TCCCTCTGGTAGGGTCATCTG |
| TUP | 95,703,234 | 95,703,259 | CAGGTATCTGTTCCTGCCTTGGCTGA |
| TUR | 95,703,208 | 95,703,228 | GGATTGCGTTGAAGCCTGAAG |
| TDF | 95,694,076 | 95,694,095 | TGGTGTGAGGGTGTCAGTTC |
| TDP | 95,694,050 | 95,694,073 | AGGGCACTGGATTCTTCCCAGGGA
|
| TDR | 95,693,996 | 95,694,015 | CAGGGTTGGTGCAGGGATAG |
PCR Primers
| Primer | Sequence |
| SU | TTGGTGAGATTGAGAGACCG |
| SD | CTCCTTGCCTTCTAGGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 54 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 260 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11588A-A1 | VGB6 | C57BL/6NTac | |
| 11588A-A6 | VGB6 | C57BL/6NTac | |
| 11588A-C2 | VGB6 | C57BL/6NTac | |
| 11588A-G10 | VGB6 | C57BL/6NTac | |
