11590   Itga2b     Status: ES cell colonies screened / QC no positives

GeneItga2b
Chromosome11
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:96601
NCBI Gene ID16399
BAC ID30C17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUFAAACTGACTGGCAAAGCTACAAG
TUPACCTACCTGGTACACCTTGCTTTCCTCC
TURGAAGGAGGTGAGGCATGGAAC
TDFCACTCTGTAGACAAAGCTAGTGTTG
TDPTCAGAGATCTGCCTGCCTCTGCCT
TDRGGTGGCACATGCCTTTAGTTC

PCR Primers

PrimerSequence
SUAGAGACAACAGGACATGGAG
SDTGCCGATCTCTGCTCTCGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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