11591 Bcl2l12 Status: Germline transmission achieved
| Gene | Bcl2l12 |
| Chromosome | 7 |
| Deletion Start | 52,252,351 |
| Deletion End | 52,246,736 |
| Deletion Size | 5,616 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,251,787 | 52,251,811 | CAGCACAAGCCATAACCATTTCAAG |
| TUP | 52,251,759 | 52,251,786 | TCTTTGTCACACCTCTTTCTAGCGGGTA |
| TUR | 52,251,730 | 52,251,751 | CCTCCTATCCAAGCACAAACAC |
| TDF | 52,247,023 | 52,247,041 | GGCAAGACAGGAGGGACAG |
| TDP | 52,246,999 | 52,247,021 | TAGAGCTCAGCGGCCCTGCTTCT |
| TDR | 52,246,932 | 52,246,955 | GACGGAGGTTAAGCAACACTGATG |
PCR Primers
| Primer | Sequence |
| SU | CTGCAAGAGTTCAAGCACTC |
| SD | TGGGTTGTGATAAAGAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11591B-F11 | VGB6 | C57BL/6NTac | |
| 11591C-A3 | VGB6 | C57BL/6NTac | |
| 11591C-C2 | VGB6 | C57BL/6NTac | |
| 11591C-F3 | VGB6 | C57BL/6NTac | |
| 11591C-G9 | VGB6 | C57BL/6NTac | |
| 11591C-H5 | VGB6 | C57BL/6NTac | |
