11594 Sh2d5 Status: Cancelled
| Gene | Sh2d5 |
| Chromosome | 4 |
| Deletion Start | 138,253,875 |
| Deletion End | 138,259,327 |
| Deletion Size | 5,453 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,254,097 | 138,254,117 | AGAAGGTGAGGCCCATGTGAC |
| TUP | 138,254,120 | 138,254,140 | AGCCAGGGCCACCTGCTGAGA |
| TUR | 138,254,142 | 138,254,163 | AGGCCGGAGTTCTCTGCTAATG |
| TDF | 138,259,163 | 138,259,183 | AAGAGCAGGACTGTGGGACTG |
| TDP | 138,259,184 | 138,259,207 | AGGGAAGGTTCCCACGGACTCTGC |
| TDR | 138,259,227 | 138,259,244 | TGCAATTCCGCCTCAGAC |
PCR Primers
| Primer | Sequence |
| SU | TGTTGCTCAGAAGCAGGCAG |
| SD | ACCAGGATGACACTAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
