11595   Jrk     Status: Cancelled

GeneJrk
Chromosome15
Deletion Start74,707,431
Deletion End74,705,716
Deletion Size1,716
Genome Build38
MGI IDMGI:106214
NCBI Gene ID16469
BAC ID286H11
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,706,76574,706,787GACTGTCTTGATGTGCGCTAATG
TUP74,706,73274,706,757CTCCCACAGAATCAAGCCTTTGGCCA
TUR74,706,68774,706,706GGTGCTGGATGCCTCTGAAG
TDF74,706,35274,706,373CACCCGCCACAACATAAATGAC
TDP74,706,32874,706,349CATAGTCAACGTGGCCCGTGCC
TDR74,706,27074,706,289CAGTGGGCCACAGTTTCCTC

PCR Primers

PrimerSequence
SUAAGAGAACAGCACTACCAGG
SDAACACCAGACACAGAACTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev349 bp-++
TUFTUR101 bp++-
TDFTDR104 bp++-
NeoFwdSD382 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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