11597   Atp5j     Status: ES cell colonies screened / QC positives

GeneAtp5j
Chromosome16
Deletion Start84,831,489
Deletion End84,827,937
Deletion Size3,553
Genome Build38
MGI IDMGI:107777
NCBI Gene ID11957
BAC ID277F7
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF84,831,45984,831,480AGGATCTTCAGGCTCTCCTCTG
TUP84,831,43484,831,457CCTTCGGTCAGCAGTCTCTGTGCA
TUR84,831,38784,831,408GTTCCTTATTAAAGGCCACAGC
TDF84,828,78484,828,807CTGCTTAGTAAGTGTCTTTGATGC
TDP84,828,75284,828,780CAAACCGTGAGGCTTAGAGACTTCCTTGA
TDR84,828,72584,828,746AGAACAGGGTTTCTGAGAGGAG

PCR Primers

PrimerSequence
SUTCTAACAGAACTCTCAGCTAC
SDAAGAGGACAGCACTGCACG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev339 bp-++
TUFTUR94 bp++-
TDFTDR83 bp++-
NeoFwdSD327 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11597A-A3VGB6C57BL/6NTac
11597A-B9VGB6C57BL/6NTac
11597A-G2VGB6C57BL/6NTac