11598 Diablo Status: ES cell colonies screened / QC no positives
| Gene | Diablo |
| Chromosome | 5 |
| Deletion Start | 123,524,117 |
| Deletion End | 123,512,475 |
| Deletion Size | 11,643 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,523,564 | 123,523,582 | TGTCGCTCGTGGAACTCAG |
| TUP | 123,523,535 | 123,523,554 | CCCTGACTCCCGAGTGCCGA |
| TUR | 123,523,480 | 123,523,497 | GAGGAAGTGCCCGCAATC |
| TDF | 123,512,874 | 123,512,893 | GCCTTTAGCACGAGTGTTCC |
| TDP | 123,512,838 | 123,512,864 | ATCCAGTCAGCCCTCCAGCATCTAACA |
| TDR | 123,512,803 | 123,512,826 | TTCCCTGACATCCTATCTCCTTTG |
PCR Primers
| Primer | Sequence |
| SU | AAGTCGGAGCGGTTGGAAG |
| SD | AAGGGTGTGGCAGGAAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 364 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
