11605 Dclre1b Status: Cancelled
| Gene | Dclre1b |
| Chromosome | 3 |
| Deletion Start | 103,809,085 |
| Deletion End | 103,802,943 |
| Deletion Size | 6,143 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,808,926 | 103,808,948 | CCTCTACTGCTCTCCCATCACTG |
| TUP | 103,808,902 | 103,808,925 | CTTGCCTCTTGCATCGTCGCCTGC |
| TUR | 103,808,852 | 103,808,872 | CCCAGATTCCTGGACTAGGTC |
| TDF | 103,803,628 | 103,803,648 | GCTATGCCTCTGATTCCACAC |
| TDP | 103,803,601 | 103,803,625 | TGTGCAGCAGTACATGAGTTCCTCC |
| TDR | 103,803,552 | 103,803,570 | GCCTCTTGAGCCTCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTTCATCCTGATCGTCC |
| SD | CCTCCAGCACCATGAATTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 513 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
