11609 Lekr1 Status: Cancelled
| Gene | Lekr1 |
| Chromosome | 3 |
| Deletion Start | 65,669,114 |
| Deletion End | 65,684,229 |
| Deletion Size | 15,116 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,669,267 | 65,669,286 | ACAGCAGAACCAGGGATGAG |
| TUP | 65,669,298 | 65,669,322 | AGGAGTAGCCACGCTGGAACGTTGA |
| TUR | 65,669,359 | 65,669,380 | TGGGACTCTAAAGCCAGAAGTG |
| TDF | 65,684,051 | 65,684,074 | TCTCTTAGCCAGGAGTTTGAACAG |
| TDP | 65,684,080 | 65,684,107 | AAGTGACAGCGAGTCCAAGAAGGCAAGG |
| TDR | 65,684,151 | 65,684,171 | CACCATGCCACCCAAATCTAC |
PCR Primers
| Primer | Sequence |
| SU | ATCCTGCTGAGCTATAATACG |
| SD | CATGTTCAAAGGCACGTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 536 bp | - | + | + |
| TUF | TUR | 114 bp | + | + | - |
| TDF | TDR | 121 bp | + | + | - |
| NeoFwd | SD | 405 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
