11611 Rnaseh2c Status: Cancelled
| Gene | Rnaseh2c |
| Chromosome | 19 |
| Deletion Start | 5,601,912 |
| Deletion End | 5,602,852 |
| Deletion Size | 941 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 5,602,168 | 5,602,189 | ATGGCTTTGCATGTCCCTCCTG |
| TUP | 5,602,190 | 5,602,212 | CAGGGCTACAGGCGTCGTTTCGC |
| TUR | 5,602,267 | 5,602,286 | TTCTCCTCCGTCACCATCAC |
| TDF | 5,602,517 | 5,602,538 | GTCCAGATGCCAAAGTGCATAG |
| TDP | 5,602,544 | 5,602,564 | TAGGTTGGCCCAGCCTCGCAG |
| TDR | 5,602,595 | 5,602,615 | GGACTGACCAAGTCTGAGGAG |
PCR Primers
| Primer | Sequence |
| SU | AGTGGAGGCACAAGAATGAG |
| SD | ATAGGCAGAGCTATCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 119 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 271 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
