| Gene | Atp6v1b1 |
| Chromosome | 6 |
| Deletion Start | 83,743,070 |
| Deletion End | 83,758,506 |
| Deletion Size | 15,437 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,743,310 | 83,743,334 | CAGAAGAACTGTGTAGTATCACCTC |
| TUP | 83,743,336 | 83,743,360 | ATGTAAAGCCTGCACATGGGTCACC |
| TUR | 83,743,391 | 83,743,411 | TCCTCTGGGTCAGCTTGAATG |
| TDF | 83,757,711 | 83,757,732 | ACAAACCTGTCTTTCCCACCTG |
| TDP | 83,757,734 | 83,757,757 | TAACAGAGGTGGCCCTGAGTGTCC |
| TDR | 83,757,759 | 83,757,778 | TGTTCCTCCACTCCCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | CACTACAGGCTCTGCTCAG |
| SD | CTGCACCTTCAACAGTTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11613A-A1 | VGB6 | C57BL/6NTac | |
| 11613B-A4 | VGB6 | C57BL/6NTac | |
