| Gene | Srsf5 |
| Chromosome | 12 |
| Deletion Start | 80,946,598 |
| Deletion End | 80,949,979 |
| Deletion Size | 3,382 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,946,886 | 80,946,908 | TCTTGTGATGAGCATGGACTCTG |
| TUP | 80,946,926 | 80,946,955 | TTCCCAGAAAGATGTTAGAAGTGGCCAAGA |
| TUR | 80,946,999 | 80,947,021 | GCTAGGGATGACTGAGAATCAAC |
| TDF | 80,949,173 | 80,949,193 | GCATGTCCTGGATTGTGAGTC |
| TDP | 80,949,199 | 80,949,221 | TAATTACCTTGGGAATTTTAATT |
| TDR | 80,949,297 | 80,949,320 | GTTGCAGGTGCCTATTATCAACAG |
PCR Primers
| Primer | Sequence |
| SU | TCTAGAAAGGTGGCTACTGG |
| SD | ACTATATACTTCAGACAGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 136 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11617A-D9 | VGB6 | C57BL/6NTac | |
| 11617A-E7 | VGB6 | C57BL/6NTac | |
