11619 Smad6 Status: Cancelled
| Gene | Smad6 |
| Chromosome | 9 |
| Deletion Start | 64,021,021 |
| Deletion End | 64,007,537 |
| Deletion Size | 13,485 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 64,020,479 | 64,020,498 | TCACGTACTCGCTGCTCAAG |
| TUP | 64,020,452 | 64,020,474 | TCAAGGAGCGTTCGCTGGACACG |
| TUR | 64,020,431 | 64,020,451 | GGACTCCACAGCCTCCAACAG |
| TDF | 64,008,139 | 64,008,162 | AAGGTCAGCAGCTATGAATTGTTC |
| TDP | 64,008,097 | 64,008,125 | AACCTTACCCAAACAGGCTTCAAGTTGGG |
| TDR | 64,008,024 | 64,008,044 | TGACCCTGACACATGGAAGAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTCCCTAAGGCTCCAAAG |
| SD | CACTAGCAAAACACATACTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 441 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
