11623 Cox17 Status: Cancelled
| Gene | Cox17 |
| Chromosome | 16 |
| Deletion Start | 38,347,086 |
| Deletion End | 38,349,327 |
| Deletion Size | 2,242 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,347,134 | 38,347,154 | CCCGAGGCTCAGGAGAAGAAG |
| TUP | 38,347,155 | 38,347,176 | CCACTGAAGCCCTGCTGTGCCT |
| TUR | 38,347,239 | 38,347,259 | AGTGGAGCCGAGCTGCTAAAG |
| TDF | 38,349,106 | 38,349,129 | GCTGGTTGGTGTAGTAGGGATTTG |
| TDP | 38,349,132 | 38,349,156 | TGTCACTCCAGCTACATCTGCACGT |
| TDR | 38,349,166 | 38,349,188 | TGGAGAATCTGTGACAGGGAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCGCCAGCGCTGACGC |
| SD | TGCCTCAAATTGTTGGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 322 bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 256 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
