11627 Mfap5 Status: Cancelled
| Gene | Mfap5 |
| Chromosome | 6 |
| Deletion Start | 122,514,327 |
| Deletion End | 122,528,450 |
| Deletion Size | 14,124 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,514,787 | 122,514,810 | GAGGGAAAGACCACCTAACACTTG |
| TUP | 122,514,813 | 122,514,836 | CCGGCGGACGCACAAAGATTTACA |
| TUR | 122,514,846 | 122,514,869 | GTGCGTACACGTAGCATATACTTC |
| TDF | 122,528,035 | 122,528,056 | TGCTCCGGTAGCTTCATTTCAG |
| TDP | 122,528,057 | 122,528,081 | CCCTCACAGACCCACTGTTAAGCCA |
| TDR | 122,528,102 | 122,528,120 | GAGCCTCGTTCTCCCTTTC |
PCR Primers
| Primer | Sequence |
| SU | TACATGCCTGCATTCCAAGC |
| SD | GCCATCCTTTATCCATCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 468 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
