11628 Eef1d Status: ES cell colonies screened / QC positives
| Gene | Eef1d |
| Chromosome | 15 |
| Deletion Start | 75,734,235 |
| Deletion End | 75,725,957 |
| Deletion Size | 8,279 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,733,871 | 75,733,889 | TCTCGGCTGACCACGTATG |
| TUP | 75,733,844 | 75,733,867 | AGACAAGCCACTCTTCGACCAGGC |
| TUR | 75,733,822 | 75,733,842 | CCTCTGACGGTAGGAGCTTTC |
| TDF | 75,726,730 | 75,726,748 | ACGCCAGTACGCAGAGAAG |
| TDP | 75,726,705 | 75,726,729 | AAGGCCAAGAAGCCCACACTGGTGG
|
| TDR | 75,726,663 | 75,726,684 | CGCCACTCACAGGTTTAACATC |
PCR Primers
| Primer | Sequence |
| SU | ACTAAGGTGTGGATGTGTGC |
| SD | GAGGATGTATCCCTCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 50 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
