| Gene | Psmb4 |
| Chromosome | 3 |
| Deletion Start | 94,881,267 |
| Deletion End | 94,878,787 |
| Deletion Size | 2,500 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,886,503 | 94,886,524 | GCGAGTCAACGACAGCACTATG |
| TUP | 94,886,473 | 94,886,497 | TGCCTCGGGAGACTACGCTGATTTC |
| TUR | 94,886,428 | 94,886,448 | GGAGGCGACTTACACCATCTG |
| TDF | 94,884,897 | 94,884,916 | GTGAGAAATGGGCTGGAAGG |
| TDP | 94,884,870 | 94,884,895 | ACAAATTCTGGAGCCAGGCATGGTGG |
| TDR | 94,884,812 | 94,884,830 | CGCCTCAAATGCAGAGTCC |
PCR Primers
| Primer | Sequence |
| SU | GCCTTGTGCATTTCTGTCAG |
| SD | GAGGTCTCAATGTGACCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 5940 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 5488 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11630A-A9 | VGB6 | C57BL/6NTac | |
| 11630A-B5 | VGB6 | C57BL/6NTac | |
| 11630A-B10 | VGB6 | C57BL/6NTac | |
| 11630A-D9 | VGB6 | C57BL/6NTac | |
