11632 Mgp Status: Cancelled
| Gene | Mgp |
| Chromosome | 6 |
| Deletion Start | 136,875,727 |
| Deletion End | 136,872,635 |
| Deletion Size | 3,093 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,874,918 | 136,874,939 | TCCTGTCTCCCTTTCTCACTTC |
| TUP | 136,874,893 | 136,874,917 | TCGGGCAGGCAGACGCTTATAAACA |
| TUR | 136,874,867 | 136,874,886 | TCGGGCAAAGCGGGAATAAG |
| TDF | 136,873,177 | 136,873,196 | GCAGAGGTGGCGAGCTAAAG |
| TDP | 136,873,140 | 136,873,168 | AGGTACTGGAACTCGACGGGAGTAGTCAT |
| TDR | 136,873,088 | 136,873,108 | GATGAACCGTCTTCCACACTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGTGTAGATACTATCTGG |
| SD | CTGACTTCACAAAGTGAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 315 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
