11633 Nox1 Status: ES cell colonies screened / QC no positives
| Gene | Nox1 |
| Chromosome | X |
| Deletion Start | 134,111,705 |
| Deletion End | 134,087,127 |
| Deletion Size | 24,579 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,111,500 | 134,111,521 | AAAGCCAACTCTAGGCCGGATG |
| TUP | 134,111,479 | 134,111,499 | AGAGAACAAAGCCAAATGATT |
| TUR | 134,111,447 | 134,111,471 | TCCTCCATTATTTGGGAACCTCAAC |
| TDF | 134,087,429 | 134,087,450 | AAGAGTGCTAGGTCCTTGTGAC |
| TDP | 134,087,406 | 134,087,428 | CCCAGCCTGCCCTGTTTCAGGAC |
| TDR | 134,087,382 | 134,087,404 | GGTGATTGGGTCCATCATAGTTC |
PCR Primers
| Primer | Sequence |
| SU | TGTGGTTGTCAGAGGCAGG |
| SD | ATTGTGCAAGTGTCAGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 344 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
