11635 Polr1d Status: ES cell colonies screened / QC no positives
| Gene | Polr1d |
| Chromosome | 5 |
| Deletion Start | 147,077,654 |
| Deletion End | 147,110,963 |
| Deletion Size | 33,310 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 147,077,968 | 147,077,989 | AGAACTGCCTCGTTGTAAGGTC |
| TUP | 147,077,990 | 147,078,008 | ACGCACCGGGCCAGCCGTC |
| TUR | 147,078,045 | 147,078,064 | CAGAGCGGAGTTGCAGTAAC |
| TDF | 147,110,184 | 147,110,206 | TCTGCTCATCTGCTAGAAGGAAC |
| TDP | 147,110,208 | 147,110,235 | TCAGCTGTAAGCGCACTGATTCACTTCG |
| TDR | 147,110,272 | 147,110,293 | GGAAAGCTCACCACAGCCTAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAGCCTATGCTCTTCAGTG |
| SD | AAAGACACCACGGATATGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 430 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 325 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
