11636 Ucp1 Status: ES cell colonies screened / QC no positives
| Gene | Ucp1 |
| Chromosome | 8 |
| Deletion Start | 83,290,569 |
| Deletion End | 83,297,972 |
| Deletion Size | 7,404 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,290,670 | 83,290,689 | ATCACCTTCCCGCTGGACAC |
| TUP | 83,290,690 | 83,290,714 | TGCCAAAGTCCGCCTTCAGGTAGAG |
| TUR | 83,290,726 | 83,290,746 | CCTGCTTGGAGAAGCCTCATC |
| TDF | 83,297,716 | 83,297,737 | TGCAGAGGAAAGATGAGTGGTG |
| TDP | 83,297,739 | 83,297,766 | CGGCACTGGGTACTTTGATCTTCCTTCC |
| TDR | 83,297,786 | 83,297,806 | GTGGTGATGGGCTGCTAAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGGTCAGGGCGCAGAAGTGC |
| SD | AAAGACCAGTTAAATACAGGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
