11640 Atp2a1 Status: ES cell colonies screened / QC no positives
| Gene | Atp2a1 |
| Chromosome | 7 |
| Deletion Start | 126,462,919 |
| Deletion End | 126,446,554 |
| Deletion Size | 16,366 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,462,126 | 126,462,146 | CTGCTTGTGCGGATTCTTCTC |
| TUP | 126,462,099 | 126,462,124 | TGGCTGCCTGCATTTCCTTTGTAAGT |
| TUR | 126,462,071 | 126,462,089 | CCCAAGGCCCACATCATAC |
| TDF | 126,447,232 | 126,447,252 | TGTCTGAGAACCAGTCCCTAC |
| TDP | 126,447,208 | 126,447,229 | CGGATGCCACCCTGGGTGAACA |
| TDR | 126,447,174 | 126,447,195 | GACATGGACAGGCAGATGGAAC |
PCR Primers
| Primer | Sequence |
| SU | AGAAACCTCTCTCTCTCCTG |
| SD | GGACACAGCCTCACAGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 424 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
