| Gene | Ypel3 |
| Chromosome | 7 |
| Deletion Start | 126,777,823 |
| Deletion End | 126,780,129 |
| Deletion Size | 2,307 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,778,339 | 126,778,358 | GGTGCTGCTGACAGGTCTTC |
| TUP | 126,778,360 | 126,778,383 | TGCTGTCGCTGACATCCACTGTGA |
| TUR | 126,778,384 | 126,778,404 | CCCAAAGTGGTCTTGCAGTTC |
| TDF | 126,779,751 | 126,779,775 | TTTGTAATCCTTGGACCTTGAAATC |
| TDP | 126,779,786 | 126,779,812 | TTCCAAATCCTCCCTGTTGGAGCTGTC |
| TDR | 126,779,824 | 126,779,844 | ACTCTGAAGCACGGGTAGTTC |
PCR Primers
| Primer | Sequence |
| SU | AGTTCAGATGCCAGCTCAGC |
| SD | GCAGCACACAGGCCTTATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 444 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 349 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11641B-A1 | VGB6 | C57BL/6NTac | |
| 11641B-B4 | VGB6 | C57BL/6NTac | |
| 11641B-G6 | VGB6 | C57BL/6NTac | |
| 11641B-F12 | VGB6 | C57BL/6NTac | |
