11642   Arc     Status: Cancelled

GeneArc
Chromosome15
Deletion Start74,672,369
Deletion End74,671,112
Deletion Size1,258
Genome Build38
MGI IDMGI:88067
NCBI Gene ID11838
BAC ID381I16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 15

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF74,672,25074,672,269GAGCTGAGATGCTGGAACAC
TUP74,672,23074,672,248TACGGAGGACCCACCGGCA
TUR74,672,19974,672,216CTCCACCTGCTTGGACAC
TDF74,671,24874,671,267CACTGCCCACAGAGGATGAG
TDP74,671,22674,671,247ACGGAGGCACTCACACCTGCTC
TDR74,671,19474,671,213GGTCCTGTCACTGGCTACTG

PCR Primers

PrimerSequence
SUAGCATAAATAGCCGCTGGTG
SDGTGATGGTCTGAGAGTGTAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev345 bp-++
TUFTUR71 bp++-
TDFTDR74 bp++-
NeoFwdSD320 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image