11647   Thrsp     Status: ES cell colonies screened / QC no positives

GeneThrsp
Chromosome7
Deletion Start97,417,500
Deletion End97,417,045
Deletion Size456
Genome Build38
MGI IDMGI:109126
NCBI Gene ID21835
BAC ID51O5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,417,47497,417,495GCTAACGAAACGCTATCCCAAG
TUP97,417,44797,417,471CTGCCTGCTGACAGTCATGGATCGG
TUR97,417,41097,417,429TCACCACCTGCTCCATGTTC
TDF97,417,19197,417,211AAACGGAGGAGGCCGAAGAAG
TDP97,417,16597,417,190ACAGGATCTCGGAGGAGCTGGACCTA
TDR97,417,13897,417,156GCAGAAGTGCAGGTGGAAC

PCR Primers

PrimerSequence
SUACACAGCTTTCCTCTAGCTG
SDGACTACAGTTCTGTCCTCTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev406 bp-++
TUFTUR86 bp++-
TDFTDR74 bp++-
NeoFwdSD335 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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