11649 Setmar Status: ES cell colonies screened / QC no positives
| Gene | Setmar |
| Chromosome | 6 |
| Deletion Start | 108,065,084 |
| Deletion End | 108,076,477 |
| Deletion Size | 11,394 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,065,120 | 108,065,139 | GCAGCATCTGAGGAGGAATC |
| TUP | 108,065,146 | 108,065,173 | CCCGACAGAACAACAAGATGTAGCGTGC |
| TUR | 108,065,182 | 108,065,202 | CACAAGCTCACAGGCAAGTTC |
| TDF | 108,075,769 | 108,075,790 | GGATGTGCTTGCATCGAAACTC |
| TDP | 108,075,795 | 108,075,817 | TGTCCCTGGCACTTGCTCTTGTC |
| TDR | 108,075,871 | 108,075,890 | GGCTTGGCGTACTTTCCTTC |
PCR Primers
| Primer | Sequence |
| SU | TTGTGGTTCTGAAATTATCAGC |
| SD | TGGGCCAAATTGCTGAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 354 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | 329 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
