11651 Zfp593 Status: ES cell colonies screened / QC no positives
| Gene | Zfp593 |
| Chromosome | 4 |
| Deletion Start | 134,245,500 |
| Deletion End | 134,244,726 |
| Deletion Size | 775 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,245,215 | 134,245,236 | GCCGCTAGAAGGTTCTGTCTTG |
| TUP | 134,245,193 | 134,245,211 | TCCCTGGGTCAACCCGCCA |
| TUR | 134,245,169 | 134,245,192 | GGATTCTGAACCCGAAGTGATAAG |
| TDF | 134,244,820 | 134,244,837 | GCCCACAGAAGTGTCCAC |
| TDP | 134,244,792 | 134,244,815 | ATCCCTGAGATGGACACGTCCACC |
| TDR | 134,244,764 | 134,244,783 | CCTCTGCCCTGCATCCTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGGAAACCGGCAAGTGAG |
| SD | TACGCTGTACCTAGCCAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 399 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
