11652 Lipt2 Status: Cancelled
| Gene | Lipt2 |
| Chromosome | 7 |
| Deletion Start | 100,159,268 |
| Deletion End | 100,160,406 |
| Deletion Size | 1,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 100,159,292 | 100,159,310 | CTGTGGTCCGGCTCGTTTG |
| TUP | 100,159,312 | 100,159,334 | CTTGGTCGGGTGCACTACTCGGA |
| TUR | 100,159,354 | 100,159,371 | TAGCCGCCGTAGCCAATG |
| TDF | 100,160,203 | 100,160,220 | CACGGCTTGGCTCTGAAC |
| TDP | 100,160,221 | 100,160,246 | TGTTCAACCGACCTCACATGGTTTGA |
| TDR | 100,160,250 | 100,160,269 | CCAGTCCACAAGGCACAATG |
PCR Primers
| Primer | Sequence |
| SU | TCGTTTCTCAAATACTCTACAC |
| SD | AGTGTCTCCATTAACAGGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 366 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
