11654 Tmub1 Status: ES cell colonies screened / QC positives
| Gene | Tmub1 |
| Chromosome | 5 |
| Deletion Start | 23,952,734 |
| Deletion End | 23,951,708 |
| Deletion Size | 1,027 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,952,506 | 23,952,524 | CCCAGCCTGAGACACAGAG |
| TUP | 23,952,483 | 23,952,504 | TCCATCTGCACAGCCAGAGCCT |
| TUR | 23,952,390 | 23,952,411 | GGCTACCTGCTCAGAGTCATTG |
| TDF | 23,952,078 | 23,952,097 | CCTCAGGACCCAGTTTCCAG |
| TDP | 23,952,054 | 23,952,076 | CCAGGAACAGCAGGTTCGGCTCA
|
| TDR | 23,952,006 | 23,952,027 | GACTGCCTAGTGTCTGAGTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCACTTGGAATCTGGTGATG |
| SD | AAGTGCCTGATCCAGAAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 315 bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 327 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
