11656 1810037I17Rik Status: Cancelled
| Gene | 1810037I17Rik |
| Chromosome | 3 |
| Deletion Start | 122,924,463 |
| Deletion End | 122,925,482 |
| Deletion Size | 1,020 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,924,752 | 122,924,773 | CGGAGTTGGAAGAGCGAAAATG |
| TUP | 122,924,777 | 122,924,798 | TTCCTTGACACCCCAGTGGCAA |
| TUR | 122,924,835 | 122,924,859 | CAGTTCGTTTGTGGTGATGAAACAG |
| TDF | 122,925,018 | 122,925,040 | GTGCGCCTGCTTAAAATGCTTAG |
| TDP | | | CCCAAAGCAAGCCTT |
| TDR | 122,925,142 | 122,925,165 | GAGAGCCAAAGAAACCCAAGATAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGCACCTTAGAAACCACC |
| SD | GTGCTACCTATTCAACTCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 148 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
