11659 Fam166b Status: ES cell colonies screened / QC no positives
| Gene | Fam166b |
| Chromosome | 4 |
| Deletion Start | 43,429,086 |
| Deletion End | 43,427,162 |
| Deletion Size | 1,925 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,428,505 | 43,428,524 | TGTCAGGATGGTGGTGTAGG |
| TUP | 43,428,472 | 43,428,498 | CTCAGAGCTATGGGATGGATCCTGTGC |
| TUR | 43,428,427 | 43,428,447 | GCCCATGCTGAACCGAAGTAG |
| TDF | 43,427,261 | 43,427,281 | GGCCACGCAGGATATAAGTTC |
| TDP | 43,427,230 | 43,427,256 | TTGGCGGTACATTTGGGCATCTTACCC |
| TDR | 43,427,188 | 43,427,210 | CCGGGAGCTGTTTCTGAGTAATG |
PCR Primers
| Primer | Sequence |
| SU | AGAGGCTTTCGCAGCTGTTC |
| SD | CCGACTGATAATGAAGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 347 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
