11660   0610037P05Rik     Status: ES cell colonies screened / QC positives

Gene0610037P05Rik
Chromosome16
Deletion Start14,317,411
Deletion End14,300,200
Deletion Size17,212
Genome Build37
MGI IDMGI:1913336
NCBI Gene ID66086
BAC ID194O14
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 16

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF14,317,18114,317,203GAGGAAGGGACTGGTATCTGTTG
TUP14,317,15114,317,178CCCTCAGGACCTCTGGGTATCTCTTAGA
TUR14,317,13014,317,148GCGCTGTCTGCTCAGTAGG
TDF14,300,86914,300,889GGGCTCAGCTAACAGGCAATC
TDP14,300,83414,300,860TTTGTTGGCCCTACAATGCTGATTGCA
TDR14,300,81114,300,831GGACTCAGGAGGAAGGACAAG

PCR Primers

PrimerSequence
SUAGCTTTTCTGCAGTGTAGCC
SDGTGGCATCCAGCAAGCGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev301 bp-++
TUFTUR74 bp++-
TDFTDR79 bp++-
NeoFwdSD255 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
11660A-E10VGB6C57BL/6NTac
11660B-D3VGB6C57BL/6NTac
11660B-D9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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