| Gene | Comt |
| Chromosome | 16 |
| Deletion Start | 18,411,954 |
| Deletion End | 18,407,816 |
| Deletion Size | 4,139 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,411,861 | 18,411,881 | TGGTGGCTATTGGTTGGTTTG |
| TUP | 18,411,840 | 18,411,860 | AGTTCGTGCAGCAGCCGGTCC |
| TUR | 18,411,801 | 18,411,818 | GGATGCGCTGCTCCTTTG |
| TDF | 18,408,691 | 18,408,712 | TGGGTTGCTTCCAGTATGTTTG |
| TDP | 18,408,654 | 18,408,682 | TGCCTTACATGTCTTACATAGTCCGGGTA |
| TDR | 18,408,627 | 18,408,648 | GCTGAGGCAGATTCATTGGTTC |
PCR Primers
| Primer | Sequence |
| SU | GCTAGAAACAGTCGGAATGC |
| SD | TTGTTCTTGGGTGCAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 423 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 361 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11664A-A6 | VGB6 | C57BL/6NTac | |
| 11664A-A10 | VGB6 | C57BL/6NTac | |
| 11664A-C4 | VGB6 | C57BL/6NTac | |
| 11664A-D4 | VGB6 | C57BL/6NTac | |
| 11664A-F5 | VGB6 | C57BL/6NTac | |
| 11664A-F11 | VGB6 | C57BL/6NTac | |
