11666 Lyl1 Status: ES cell colonies screened / QC no positives
| Gene | Lyl1 |
| Chromosome | 8 |
| Deletion Start | 84,702,649 |
| Deletion End | 84,704,352 |
| Deletion Size | 1,704 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,703,107 | 84,703,129 | GACCCTTCAGCATCTTCCCTAAC |
| TUP | 84,703,136 | 84,703,161 | CTGAAGCGCAGACCAAGCCATAGTGA |
| TUR | 84,703,163 | 84,703,181 | CACCGTCAGCCAAGTCCAG |
| TDF | 84,703,692 | 84,703,716 | ACACTTGCTAGGAATGAAACCACAG |
| TDP | 84,703,719 | 84,703,743 | TGGACCTCTCAAGGGATCACATCGC |
| TDR | 84,703,761 | 84,703,781 | ACATCGGAACTGCTGAGTCTG |
PCR Primers
| Primer | Sequence |
| SU | ATTCCCAGCTGCAGGTAGG |
| SD | ACACTTGTCTAGGGTCTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 462 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 357 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
