| Gene | Usp27x |
| Chromosome | X |
| Deletion Start | 7,374,858 |
| Deletion End | 7,373,475 |
| Deletion Size | 1,384 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 7,374,352 | 7,374,370 | TGCCCACGAGTTCCTCATC |
| TUP | 7,374,326 | 7,374,349 | TGCGCTAGATGTCCTGCATAGGCA |
| TUR | 7,374,295 | 7,374,312 | GGCCACCTTGCCAACATC |
| TDF | 7,374,104 | 7,374,125 | GGAGGGAGAGCAGTTTGAATGG |
| TDP | 7,374,079 | 7,374,103 | CGAAAGCCACATCCCAGGCATCACC |
| TDR | 7,374,060 | 7,374,077 | GCAAGCAGTCCGTGAGAG |
PCR Primers
| Primer | Sequence |
| SU | CCTGGATGTGGATTTTGCTG |
| SD | TCAATTGAAGCTGAGACACCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11667A-D3 | VGB6 | C57BL/6NTac | |
| 11667A-D7 | VGB6 | C57BL/6NTac | |
| 11667A-E7 | VGB6 | C57BL/6NTac | |
| 11667A-H10 | VGB6 | C57BL/6NTac | |
| 11667B-A8 | VGB6 | C57BL/6NTac | |
| 11667B-C10 | VGB6 | C57BL/6NTac | |
