11672 Aspdh Status: ES cell colonies screened / QC no positives
| Gene | Aspdh |
| Chromosome | 7 |
| Deletion Start | 44,465,455 |
| Deletion End | 44,467,703 |
| Deletion Size | 2,249 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,466,368 | 44,466,386 | TCAGCCAGGGTAAGCAGTG |
| TUP | 44,466,390 | 44,466,413 | TCCCTCCAAACCCTGAGGACCTGG |
| TUR | 44,466,426 | 44,466,445 | GGGCCTTGGAGAAGAGTCAG |
| TDF | 44,467,353 | 44,467,371 | GGGTCCCTTGGCGTTTGTG |
| TDP | 44,467,373 | 44,467,395 | TCGGCAGAGCTCACATGTTGGGT |
| TDR | 44,467,424 | 44,467,447 | GCCTGGGAGTGAGAACTAAGAATG |
PCR Primers
| Primer | Sequence |
| SU | GAATTTTCTACCTCAGCAGG |
| SD | AACGAGTGATCTCGCGATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 279 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
