11673 Mina Status: ES cell colonies screened / QC no positives
| Gene | Mina |
| Chromosome | 16 |
| Deletion Start | 59,475,522 |
| Deletion End | 59,491,901 |
| Deletion Size | 16,380 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,476,457 | 59,476,479 | ATGGTAAGGATCTCTGTGCTGTC |
| TUP | 59,476,484 | 59,476,512 | TGCTGTACTTGAAGGCACATGGTAACACC |
| TUR | 59,476,516 | 59,476,538 | GGGAAGATAACCGGAAGTATCAC |
| TDF | 59,491,770 | 59,491,791 | TGTGGATGCTCTGAAGCAAATC |
| TDP | 59,491,792 | 59,491,815 | TGGTGCGGGTCACCAATTCGTGTT |
| TDR | 59,491,819 | 59,491,843 | CTTCATCTGTGTCAAGTTTCAGGTC |
PCR Primers
| Primer | Sequence |
| SU | GTGCTTGTAGCAATAAGATAGT |
| SD | ATGACATGTGTACTGCAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 372 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 339 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
