11674 Cdc20 Status: ES cell colonies screened / QC positives
| Gene | Cdc20 |
| Chromosome | 4 |
| Deletion Start | 118,109,723 |
| Deletion End | 118,105,548 |
| Deletion Size | 4,176 |
| Genome Build | 37 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,109,155 | 118,109,175 | TTTGATGTGGAGGAAGCCAAG |
| TUP | 118,109,131 | 118,109,153 | TCCTCAGGCTCAGCGGCAAACCT |
| TUR | 118,109,088 | 118,109,107 | GCCTCGATGATGTCACTCAC |
| TDF | 118,106,072 | 118,106,093 | GCTCAAAGGTAGGTGGCAATAG |
| TDP | 118,106,045 | 118,106,070 | AAGCCAGGCAGAAAGGCCACATAGTG
|
| TDR | 118,105,988 | 118,106,008 | CCCAGGCAAGTCATATCAGTC |
PCR Primers
| Primer | Sequence |
| SU | TTAAGAACAACAGTCCGGCG |
| SD | AGTGCATGCGGTAATTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TCATTCTCAGTATTGTTTTGCC |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11674A-A10 | VGB6 | C57BL/6NTac | |
| 11674A-D2 | VGB6 | C57BL/6NTac | |
| 11674A-C9 | VGB6 | C57BL/6NTac | |
