11676 Pigy Status: ES cell colonies screened / QC no positives
| Gene | Pigy |
| Chromosome | 6 |
| Deletion Start | 57,692,002 |
| Deletion End | 57,689,688 |
| Deletion Size | 2,315 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,691,649 | 57,691,668 | TTTTCTCTGGCTCGCCATTG |
| TUP | 57,691,617 | 57,691,646 | TGCTCTTTACAATCTCTCCTGGAGGCTTTA |
| TUR | 57,691,590 | 57,691,610 | GCCAGTCTCAGAAACCAAGTC |
| TDF | 57,690,001 | 57,690,023 | GGATTGGCCTATGCTTCTAAGAC |
| TDP | 57,689,982 | 57,689,999 | AAAGTTACTGGAGGACTG |
| TDR | 57,689,933 | 57,689,956 | TGACGCTCAATAAATGGCACTATG |
PCR Primers
| Primer | Sequence |
| SU | GACACCTGAATGAGTACTGC |
| SD | GTTTTAGACCTTTCTGGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 374 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
