11678 Crebzf Status: ES cell colonies screened / QC no positives
| Gene | Crebzf |
| Chromosome | 7 |
| Deletion Start | 90,443,001 |
| Deletion End | 90,444,093 |
| Deletion Size | 1,093 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,443,299 | 90,443,318 | CATGGTCCCCAAGGAAGAGC |
| TUP | 90,443,319 | 90,443,344 | CGGAGGACATGGACTTTCTCTCCGGG |
| TUR | 90,443,345 | 90,443,364 | AGCAGATCGGCCAGCTCCAG |
| TDF | 90,443,772 | 90,443,791 | AGAATCGGGAGCTGGGCAAG |
| TDP | 90,443,792 | 90,443,811 | CGCGTGCAAGCACTGCAGGA |
| TDR | 90,443,853 | 90,443,870 | TCAGCAGGCGAGCTAGTC |
PCR Primers
| Primer | Sequence |
| SU | CACTCGGAATCTCGCGATAC |
| SD | GGAGTTGGTTACAGTGTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 412 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 252 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
