11682 Sat2 Status: ES cell colonies screened / QC one positive
| Gene | Sat2 |
| Chromosome | 11 |
| Deletion Start | 69,622,252 |
| Deletion End | 69,623,669 |
| Deletion Size | 1,418 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,622,873 | 69,622,894 | GGGCTATGGGCTGTACTACTTC |
| TUP | 69,622,901 | 69,622,923 | AGCACATGGACCGGACGAAACGT |
| TUR | 69,622,931 | 69,622,952 | GCGGCATCACATAGATGTCTTC |
| TDF | 69,623,462 | 69,623,482 | CCTGTGCAGTAAGCGTATGTC |
| TDP | 69,623,487 | 69,623,511 | ACAGGTTGCCCTGAATAAGGGTTGC |
| TDR | 69,623,536 | 69,623,556 | CACGGCCTTCTTGTTCCAATC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTGGCAGAAGAGACCAC |
| SD | AGTCCTTGAGGCCTCCATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 318 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 379 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
