11683 2810405K02Rik Status: Cancelled
| Gene | 2810405K02Rik |
| Chromosome | 4 |
| Deletion Start | 154,899,002 |
| Deletion End | 154,896,529 |
| Deletion Size | 2,474 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 154,898,473 | 154,898,495 | TGGTGGTTACTTCTCAGGAGGTG |
| TUP | 154,898,450 | 154,898,472 | CGACTCTTCCCGGACTCAGAGCC |
| TUR | 154,898,424 | 154,898,445 | GGCCTAGAAAGGGAGAGGTCAG |
| TDF | 154,897,436 | 154,897,453 | TGACCTGAGTCCCGTTCC |
| TDP | 154,897,405 | 154,897,431 | TTTGCAGGCTAAAGCTGTTGGTATCCA |
| TDR | 154,897,361 | 154,897,379 | CCAGCAGCCCTCCACTTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTGTATACTAATATCAGG |
| SD | AGGAAGATGACACTTCGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 319 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 297 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
