11685   Pcbp1     Status: ES cell colonies screened / QC no positives

GenePcbp1
Chromosome6
Deletion Start
Deletion End
Deletion Size
Genome Build37
MGI IDMGI:1345635
NCBI Gene ID23983
BAC ID362B9
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Sequence Downloads and Strategy Information

  1. 2,000 Bases Upstream (not yet available)
  2. 2,000 Bases Downstream (not yet available)
  3. Primer Sequences
  4. Cassette
  5. Design Diagram
  6. PCR Conditions
  7. Genotyping Strategy

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF86,525,89386,525,911ACGCCGGTGTGACTGAAAG
TUP86,525,86486,525,889ACTCAACGTGACTCTCACCATTCGGC
TUR86,525,82686,525,843CCCGATGATGCTGCCTAC
TDF86,525,33586,525,353CCAGCTCGCCAGTCATCTG
TDP86,525,31386,525,332CGGGCGGCCAAGATCGCTGC
TDR86,525,21586,525,234TCCAGCGGAGAAATGGTGTG

PCR Primers

PrimerSequence
SUCTGGCGCAGTCTTGTGACC
SDATGGATCCCAGCATTAACGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR86 bp++-
TDFTDR139 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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