11686 Kdm6b Status: ES cell colonies screened / QC no positives
| Gene | Kdm6b |
| Chromosome | 11 |
| Deletion Start | 69,408,159 |
| Deletion End | 69,399,697 |
| Deletion Size | 8,463 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,407,295 | 69,407,314 | CTTTCATGCCGGTTCCTGTC |
| TUP | 69,407,271 | 69,407,294 | AGCACAGAGCCAAGGTCCTGCCTC |
| TUR | 69,407,221 | 69,407,241 | CTCAGCCTCACTCACCTCAAG |
| TDF | 69,400,373 | 69,400,393 | ATCTGCCATCCTCCAGCCTTG |
| TDP | 69,400,340 | 69,400,364 | CTGGAGCCTTGTCTCAACACCCACC |
| TDR | 69,400,284 | 69,400,305 | TGTACCTGACAGTGCTTCATTG |
PCR Primers
| Primer | Sequence |
| SU | AGAATTGGTTGCAGAAGGCC |
| SD | CGTCTATGTACACAACGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 374 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
