11687 6530418L21Rik Status: ES cell colonies screened / QC no positives
| Gene | 6530418L21Rik |
| Chromosome | 3 |
| Deletion Start | 105,704,754 |
| Deletion End | 105,717,222 |
| Deletion Size | 12,469 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,704,777 | 105,704,795 | GGAGAGCAAGGACATGGAC |
| TUP | 105,704,796 | 105,704,818 | TGCTATCTGCGTCGCCTCAAACA |
| TUR | 105,704,861 | 105,704,879 | CTAGGTCTGGGTGGAGCAG |
| TDF | 105,717,003 | 105,717,021 | AGAAGCCTGGCTGGATGAC |
| TDP | 105,717,023 | 105,717,046 | CCCATGGTCTCTGAGTCACGAGCA |
| TDR | 105,717,081 | 105,717,100 | GAACCGTCCTGAGCCCTTAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGAGTTCTCATTGGCTGC |
| SD | ATTATTGTCACATCCAGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | 366 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
