| Gene | Lipa |
| Chromosome | 19 |
| Deletion Start | 34,524,839 |
| Deletion End | 34,493,960 |
| Deletion Size | 30,880 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,524,767 | 34,524,786 | GCCCACGGGAACTGTATCAG |
| TUP | 34,524,736 | 34,524,765 | TGTGGATCCTGAAGTCAACATGAATGTGGT |
| TUR | 34,524,640 | 34,524,663 | AGCGGTAAAGAGCTTAGGGTTGTG |
| TDF | 34,494,552 | 34,494,572 | GCATTCAGAGGGCATGAAGAG |
| TDP | 34,494,529 | 34,494,551 | ATGAGCCCACAGTGCCTCCCTGT |
| TDR | 34,494,498 | 34,494,520 | GATGTGGAGTCAAGGCAGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | TGTCTGGAGGCCTCTTAATG |
| SD | TCACTGGTTGTGGACGCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 322 bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11689B-B3 | VGB6 | C57BL/6NTac | |
| 11689B-E5 | VGB6 | C57BL/6NTac | |
| 11689B-H9 | VGB6 | C57BL/6NTac | |
| 11689C-C10 | VGB6 | C57BL/6NTac | |
| 11689C-H7 | VGB6 | C57BL/6NTac | |
| 11689C-A10 | VGB6 | C57BL/6NTac | |
| 11689C-C2 | VGB6 | C57BL/6NTac | |
