11690 Cldn17 Status: Cancelled
| Gene | Cldn17 |
| Chromosome | 16 |
| Deletion Start | 88,506,836 |
| Deletion End | 88,506,144 |
| Deletion Size | 693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,506,785 | 88,506,807 | AGTTCTTGGATTCTTCGGTTTGG |
| TUP | 88,506,758 | 88,506,783 | TGGGACGATTGGGACAACACTTCTGC |
| TUR | 88,506,726 | 88,506,744 | GCTGCCGATGAAAGCTGAC |
| TDF | 88,506,359 | 88,506,376 | CAGGGCAGAAGCGAGAAC |
| TDP | 88,506,334 | 88,506,357 | TGGAGGAGCACTCTTCCTTGGCTG |
| TDR | 88,506,310 | 88,506,331 | ATGAAGAGGACAGCAGCGGTTG |
PCR Primers
| Primer | Sequence |
| SU | ACAGAAATGAAGGCGTGTGC |
| SD | AGTTCAACAAGAGTGAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 336 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 288 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
